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How to tell the purity of caffeic acid?

2023-04-28

  

The purity of caffeic acid can be determined using various analytical techniques, such as:

High-performance liquid chromatography (HPLC): This technique involves separating the components of a mixture based on their interactions with a stationary phase and a mobile phase.  The percentage of pure caffeic acid can be calculated based on the area of the peak obtained from the HPLC analysis.


Mass spectrometry (MS): This technique involves ionizing the molecules of the compound and then measuring the mass-to-charge ratio of the resulting ions. The purity of caffeic acid can be determined by comparing the mass spectrum obtained with that of the pure compound.

Infrared (IR) spectroscopy: This technique involves passing infrared radiation through the sample and measuring the energy absorbed by the sample at different wavelengths. The purity of caffeic acid can be determined by comparing the IR spectrum obtained with that of the pure compound.

Nuclear magnetic resonance (NMR) spectroscopy: This technique involves applying a strong magnetic field to the sample and measuring the energy absorbed by the nuclei of the atoms in the compound. The purity of caffeic acid can be determined by comparing the NMR spectrum obtained with that of the pure compound.

It is recommended to consult a qualified analytical chemist or laboratory to perform these tests and accurately determine the purity of caffeic acid.