Caffeic Acid Powder Price,detected by high performance liquid chromatography

High Performance Liquid Chromatography (HPLC) has various advantages in the analysis of caffeic acid, which mainly include the following aspects:

High Separation Efficiency: Caffeic acid samples may contain multiple components. HPLC can effectively separate caffeic acid from other coexisting impurities or compounds with similar structures by utilizing the differences in the partition coefficients of different substances between the stationary phase and the mobile phase, thus enabling precise analysis of caffeic acid. Even for structural isomers with similar structures, HPLC can achieve baseline separation by selecting appropriate chromatographic columns and mobile phase conditions, providing a prerequisite for accurately determining the content of caffeic acid.

Fast Analysis Speed: Modern high performance liquid chromatographs are equipped with high-efficiency infusion pumps and chromatographic columns for rapid separation, which can complete the separation and detection of caffeic acid within a relatively short period. Generally, an analysis process only takes a few minutes to dozens of minutes. Compared with some other traditional analysis methods, such as the gravimetric method and the titration method, the analysis efficiency is greatly improved, making it suitable for the rapid detection of a large number of samples.

High Detection Sensitivity: HPLC can be coupled with a variety of highly sensitive detectors, such as ultraviolet-visible detectors, fluorescence detectors, electrochemical detectors, etc. Taking the ultraviolet-visible detector as an example, it has a high response to caffeic acid and can detect the signal of caffeic acid at low concentrations. Its detection limit can usually reach the level of micrograms per milliliter or even nanograms per milliliter, which can meet the analysis requirements of samples with a low content of this component. For example, it can be used for the trace analysis of caffeic acid in some plant extracts or complex biological samples.

Accurate Quantitative Analysis: By using standard substances for calibration, HPLC can establish an accurate quantitative analysis method. Within an appropriate concentration range, the peak area or peak height has a good linear relationship with the concentration of caffeic acid, and the correlation coefficient can usually reach above 0.99. At the same time, HPLC has good repeatability, high precision of the analysis results, and the relative standard deviation (RSD) can generally be controlled within a small range, thus ensuring the accuracy and reliability of quantitative analysis. It can be used for the precise determination of the content of caffeic acid and quality control.

Wide Applicability to Samples: HPLC has relatively low requirements for the form and properties of samples. Whether it is a liquid sample or a solid sample, as long as it can be dissolved in an appropriate solvent to prepare a solution, it can be analyzed. For caffeic acid, regardless of whether it exists in different matrices such as coffee beverages, plant tissue extracts, or pharmaceuticals, it can be analyzed by HPLC after appropriate sample pretreatment. There is no need for complex pretreatment steps such as derivatization of the sample. The operation is simple, and it can retain the original properties of caffeic acid to the greatest extent.