The content detection method of hydroxytyrosol manufacturers

The content detection methods of hydroxytyrosol mainly include high-performance liquid chromatography, gas chromatography, capillary electrophoresis, etc. The specific introductions are as follows:

I. High-performance Liquid Chromatography (HPLC)

Principle: Utilize the differences in the distribution coefficients of different substances between the stationary phase and the mobile phase to separate and quantitatively analyze hydroxytyrosol. Inject the sample into the high-performance liquid chromatograph, and after separation by the chromatographic column, detect the peak area or peak height of hydroxytyrosol using an ultraviolet detector or a diode array detector, and compare it with the peak area or peak height of the standard substance to calculate the content of hydroxytyrosol in the sample.

Advantages: It has the advantages of high separation efficiency, fast analysis speed, high detection sensitivity, and good accuracy. It can simultaneously separate and determine multiple compounds and is suitable for the content detection of hydroxytyrosol in complex samples.

Disadvantages: Professional instrumentation is required, the instruments are expensive, the maintenance cost is high, and the technical requirements for the operators are also high.

II. Gas Chromatography (GC)

Principle: After vaporizing the sample, it enters the gas chromatographic column driven by the carrier gas, and is separated according to the differences in the distribution coefficients of different substances between the stationary phase and the mobile phase, and then detected using a flame ionization detector (FID) or a mass spectrometry detector (MS), etc. For the detection of hydroxytyrosol, it is usually necessary to derivatize it first to convert it into a volatile derivative suitable for gas chromatographic analysis.

Advantages: It has good separation effect, high sensitivity, and fast analysis speed, and can be used for the separation and quantitative analysis of multiple compounds. For some compounds with strong volatility, gas chromatography has unique advantages.

Disadvantages: The sample needs to be derivatized, and the operation steps are relatively cumbersome. At the same time, gas chromatography has high requirements for the volatility of the sample. For some compounds with strong polarity and poor volatility, special treatment may be required for analysis.

III. Capillary Electrophoresis (CE)

Principle: Separate and analyze based on the differences in the migration speeds of different charged particles in an electric field. In a capillary electrophoresis instrument, the sample is driven by an electric field in a buffer solution, separated through a capillary column, and then detected using an ultraviolet detector or a laser-induced fluorescence detector, etc. Hydroxytyrosol will migrate at different speeds according to its properties such as charge and size in the electric field, thus achieving separation and quantitative analysis.

Advantages: It has the advantages of high separation efficiency, small sample consumption, fast analysis speed, and low cost, and is suitable for the separation and analysis of various types of compounds, especially for the analysis of trace components in biological samples.

Disadvantages: The detection sensitivity is relatively low, and it may be difficult to detect some samples with low content. At the same time, the separation effect of capillary electrophoresis is greatly affected by factors such as the composition of the buffer solution, pH value, and electric field strength, and optimization and control are required.